Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands
Role of cell-ECM signaling in mammary gland development and breast cancer development and progression/metastasis. Integrated signaling between receptor protein tyrosine kinase and focal adhesion kinase (FAK). FAK-mediated signaling.
siRNA and shRNA libraries. Retro- and lenti-viral transduction of MMEC and breast tumor cell lines. 2D and 3D MEC cultures. Live cell imaging (spectral imaging, TIRF microscopy, confocal, DIC) followed by quantitative image analysis. Fluorescence and bioluminescence analysis of breast cancer progression. Conditional expression of DN and DA target genes in orthotopic breast tumor models. Intravital imaging of breast tumor cell migration in primary tumor. Gene expression profilling.
Conditional knockouts for FAK and p53. Mammary gland reconstitution models. Orthotopic breast tumor/metastasis models. 2D and 3D migration/invasion models. 3D branching morphogenesis models. Models for focal adhesion dynamics.