Xose Bustelo

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Xose
Bustelo
+34 923 29 4802
Salamanca Cancer Research Center / Centro de Investigacion del Cancer de Salamanca
2006
Our research is focused on the functional characterization of signal transduction molecules with oncogenic potential and, particularly, on the functional analysis of oncoproteins specialized in connecting the stimulation of membrane receptors with signaling routes that lead to both cytoskeletal change and mitogenic processes. Within this general objetive, the current research of our laboratory is aimed at solving the following biological issues: (1) Functional characterization of the Vav oncoprotein family, a group of signal transduction molecules that work as phosphorylation-dependent GDP/GTP exchange factors for the GTPases of the Rho/Rac family. (2) Functional analysis of specific members of Rho/Rac family of GTPases. (3)Characterization of intracellular positive and negative regulators of Rho/Rac GTPases. (4) Study of the cross-talk between cytoskeletal routes and other biological processes. (5) Characterization of the role of these signaling routes in cancer, paying attention to both intrinsic (proliferation, survival, metastasis) and extrinsic (angiogenesis, inflammatory response) pathways that affect the final fitness of tumors in vivo. (6) Characterization of the role of these signal transduction pathways in other high incidence health problems such as cardiovascular disease and the metabolic syndrome. (7) Development of new therapeutic avenues to treat those diseases using molecular targets belonging to those signaling routes. To achieve these aims, our laboratory utilizes a quite diverse collection of experimental tools, including biochemical analysis (i.e., determination of enzyme activities, post-translational modifications, protein-protein interactions), structural biology (i.e., determination of the three-dimensional structure of proteins), cell biology, cell signaling (i.e., characterization of the signaling routes used by the oncoproteins under study) transgenic and knockout mice, genetic models available in invertebrate species (Drosophila, yeast) as well as genomics, proteomics, and cellomics techniques.
Signal transduction, cytoskeleton, DNA microarrays, proteomics, videomicroscopy, confocal microscopy, cell lines, primary epithelial cells, epithelial tumor biology, cardiovascular techniques, xenograft, KO and transgenic mice
Human pathologies, cell lines, knockout and knock-in mouse models.